{"release":{"schemaVersion":"maha-epistemic-release/1.0","releaseId":"epirelease_0eb6f5296fba403b8a034b3b79d25956","releaseKind":"initial","status":"active","recordId":"urn:maha:record:longevity-metabolism-lc3-turnover-assays","domainSlug":"longevity-metabolism","targetSha256":"sha256:d41e23431b7c29de0a5301bdaa86aef0a388ad6492f39a4ce6a75bb1d90dc62d","canonicalPath":"/knowledge/longevity-metabolism/measurements/longevity-metabolism-lc3-turnover-assays","canonicalVersion":"1.0.0","supersedesReleaseId":null,"approvals":[{"scope":"boundary-adequacy","reviewId":"epireview_8b27c20253c74971bf3e9c4cfa461532","reviewSha256":"sha256:d95d2679092a1111e0a376e77e53789c14bd7f54a27d4dff85d9d00e0df019e9","reviewedAt":"2026-08-25T09:34:35.851Z","reviewerKind":"internal-editorial","reviewMethod":"Deterministic exact-hash checks, public source-resolution evidence, source-to-claim audit, unsupported-inference audit, and AI-assisted internal editorial inspection. No external reviewer participated."},{"scope":"domain-fidelity","reviewId":"epireview_072f3c4d2fb8484288ef1e574a9e7960","reviewSha256":"sha256:4715a8618ebbbb23bef60cbb04d873dfcefd2b9fdeb990dcc1451d291186c732","reviewedAt":"2026-08-25T09:34:35.656Z","reviewerKind":"internal-editorial","reviewMethod":"Deterministic exact-hash checks, public source-resolution evidence, source-to-claim audit, unsupported-inference audit, and AI-assisted internal editorial inspection. No external reviewer participated."},{"scope":"rights-and-locator","reviewId":"epireview_1e998456cb934405968773721745b333","reviewSha256":"sha256:1ed6acb34389c6c18f3e92a0a37ff0816a2cb7da2d7a58f85bdd9186bbfa2431","reviewedAt":"2026-08-25T09:34:35.935Z","reviewerKind":"internal-editorial","reviewMethod":"Deterministic exact-hash checks, public source-resolution evidence, source-to-claim audit, unsupported-inference audit, and AI-assisted internal editorial inspection. No external reviewer participated."},{"scope":"source-fidelity","reviewId":"epireview_98cc8ae6de334a488e68a6692189f08f","reviewSha256":"sha256:1f81b37cc8f8954529d1d5fed8d35fa4128a9c7c380089c8b98417293cf83b50","reviewedAt":"2026-08-25T09:34:35.558Z","reviewerKind":"internal-editorial","reviewMethod":"Deterministic exact-hash checks, public source-resolution evidence, source-to-claim audit, unsupported-inference audit, and AI-assisted internal editorial inspection. No external reviewer participated."}],"assuranceTier":"internally-reviewed-canonical","releaseAuthority":{"authoritySha256":"sha256:38616eafd13c4ad603a3a3a9e445f3862fb29b54385aa571c197a2923e302785","attribution":"withheld-by-consent"},"publicChangeSummary":"Initial canonical publication in the eight-domain internal-review throughput canary.","recordSha256":"sha256:2a8c6710964b2740c53ac318b45d51eb423493343a901279e2866ff16412bb4a","gateDecision":{"reasons":[],"recordId":"urn:maha:record:longevity-metabolism-lc3-turnover-assays","publicEligible":true,"evaluatedAgainst":"maha-epistemic/1.0"},"releasedAt":"2026-08-25T09:35:23.253Z","releaseSha256":"sha256:d673492705e09ff645920d01ee86825e16459e798041649df35a99560139bbe3","withdrawal":null},"provenance":{"schemaVersion":"maha-epistemic/1.0","evidencePolicyVersion":"mps/0.1","recordId":"urn:maha:record:longevity-metabolism-lc3-turnover-assays","canonicalPath":"/knowledge/longevity-metabolism/measurements/longevity-metabolism-lc3-turnover-assays","contentHash":"sha256:2a8c6710964b2740c53ac318b45d51eb423493343a901279e2866ff16412bb4a","generatedAt":"2026-08-25T09:35:23.253Z","publicationDecision":{"recordId":"urn:maha:record:longevity-metabolism-lc3-turnover-assays","publicEligible":true,"evaluatedAgainst":"maha-epistemic/1.0","reasons":[]},"claims":[{"id":"urn:maha:claim:longevity-metabolism-lc3-turnover-assays","scope":"Limited to Assay interpretation sections for LC3, SQSTM1/p62, lysosomal inhibition, and autophagic flux. in “Guidelines for the use and interpretation of assays for monitoring autophagy”; this candidate records the concept boundary and does not pool results from uncited systems or studies.","boundary":"LC3 turnover assays does not by itself establish system-level performance, safety, manufacturability, scalability, economic advantage, clinical benefit, or deployment readiness.","claimKind":"observation","sourceIds":["source-longevity-metabolism-autophagy-guidelines"],"statement":"The cited source supports treating lc3 turnover assays as a distinct measurement within the stated longevity and metabolism scope.","replication":{"asOfDate":"2026-08-24","assessment":"Independent replication and cross-platform transfer have not been compiled for this candidate; the evidence maturity refers only to the bounded source contract.","independentReplicationCount":null},"uncertainty":{"kind":"qualitative","statement":"No cross-source quantitative interval is asserted. Definitions, operating conditions, samples, instruments, and outcome measures must be checked against the exact cited locator during review."},"evidenceMaturity":"single-study"}],"sources":[{"id":"source-longevity-metabolism-autophagy-guidelines","url":"https://pubmed.ncbi.nlm.nih.gov/22966490/","title":"Guidelines for the use and interpretation of assays for monitoring autophagy","rights":{"note":"The candidate uses original boundary language and a short paraphrase linked to the cited source. No source passage, figure, or table is reproduced.","basis":"citation-with-paraphrase","quotationUsed":false},"authors":["Daniel J. Klionsky","et al."],"boundary":"No single marker establishes autophagy rate, organismal benefit, or a longevity outcome.","publisher":"Autophagy","establishes":"The guidelines distinguish static autophagosome measurements from dynamic flux and describe assay-specific interpretation limits.","identifiers":[{"value":"10.4161/auto.19496","scheme":"doi"}],"publishedAt":"2012-04-01","exactLocator":"Assay interpretation sections for LC3, SQSTM1/p62, lysosomal inhibition, and autophagic flux."}],"reviewEvents":[{"reviewId":"epireview_98cc8ae6de334a488e68a6692189f08f","scope":"source-fidelity","targetSha256":"sha256:d41e23431b7c29de0a5301bdaa86aef0a388ad6492f39a4ce6a75bb1d90dc62d","reviewedAt":"2026-08-25T09:34:35.558Z","verdict":"approve","supersedesReviewId":null},{"reviewId":"epireview_072f3c4d2fb8484288ef1e574a9e7960","scope":"domain-fidelity","targetSha256":"sha256:d41e23431b7c29de0a5301bdaa86aef0a388ad6492f39a4ce6a75bb1d90dc62d","reviewedAt":"2026-08-25T09:34:35.656Z","verdict":"approve","supersedesReviewId":null},{"reviewId":"epireview_8b27c20253c74971bf3e9c4cfa461532","scope":"boundary-adequacy","targetSha256":"sha256:d41e23431b7c29de0a5301bdaa86aef0a388ad6492f39a4ce6a75bb1d90dc62d","reviewedAt":"2026-08-25T09:34:35.851Z","verdict":"approve","supersedesReviewId":null},{"reviewId":"epireview_1e998456cb934405968773721745b333","scope":"rights-and-locator","targetSha256":"sha256:d41e23431b7c29de0a5301bdaa86aef0a388ad6492f39a4ce6a75bb1d90dc62d","reviewedAt":"2026-08-25T09:34:35.935Z","verdict":"approve","supersedesReviewId":null}]},"privacyBoundary":"Operational actor fingerprints, bearer credentials, private reviewer profiles, affiliations, conflicts, and non-consented authority identity fields are excluded."}