Bounded definition
The cited biochemical study reconstitutes programmable Cas9 cleavage and shows that a designed single guide can direct sequence-specific cutting adjacent to a compatible motif. Within this page, that proposition is limited to The constructs, biological systems, protocols, assays, datasets, and comparisons reported in A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Definition and evidence boundary
RNA-guided DNA cleavage using a Cas9 nuclease and target-complementary guide sequence. The bounded proposition retained by the canonical record is: The cited biochemical study reconstitutes programmable Cas9 cleavage and shows that a designed single guide can direct sequence-specific cutting adjacent to a compatible motif.
The applicable scope is The constructs, biological systems, protocols, assays, datasets, and comparisons reported in A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. This definition must not be generalized beyond the cited source and exact record boundary.
Claims: urn:maha:claim:crispr-cas9-nuclease-editing
Mechanism and technical context
The study reconstitutes RNA-programmed Cas9 cleavage in vitro and shows that a designed single-guide RNA can direct sequence-specific DNA cleavage adjacent to an appropriate motif. This is the source-bound technical context for the record; no uncited mechanism is added by the compiler.
Biochemical cleavage is not equivalent to efficient, specific, or safe genome editing in a cell or organism. The mechanism or method is therefore presented as one component of a larger system, not as evidence for every downstream outcome.
Claims: urn:maha:claim:crispr-cas9-nuclease-editing
How to interpret the evidence
There is no universal effect estimate for this method; numerical results remain attached to the source experiment, biological system, assay, and analysis choices. The evidence maturity recorded here is single study, and the claim kind is empirical claim.
This candidate records one bounded source package. Independent replications and contradictory results must be compiled separately before evidence maturity is upgraded. An in-vitro nuclease mechanism does not establish editing efficiency, specificity, delivery, safety, or phenotype in cells or organisms. These qualifications travel with the claim whenever it is reused.
Claims: urn:maha:claim:crispr-cas9-nuclease-editing
What the source supports and what remains unknown
The inspected source supports exactly this: The study reconstitutes RNA-programmed Cas9 cleavage in vitro and shows that a designed single-guide RNA can direct sequence-specific DNA cleavage adjacent to an appropriate motif. It was read at Abstract; Figures 1–5; supplementary materials describing guide design and cleavage assays.
What remains unknown is everything outside that locator. Biochemical cleavage is not equivalent to efficient, specific, or safe genome editing in a cell or organism. No quantity, comparison, or downstream outcome is established here unless a separately scoped record measures it.
Claims: urn:maha:claim:crispr-cas9-nuclease-editing
Source identity, locator, and reuse boundary
The bound source is “A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity” by Martin Jinek, Krzysztof Chylinski, Ines Fonfara, Michael Hauer, Jennifer A. Doudna, Emmanuelle Charpentier, published by Science on 2012-06-28; its declared stable identity is doi:10.1126/science.1225829.
The inspected-content locator is Abstract; Figures 1–5; supplementary materials describing guide design and cleavage assays. Reuse is limited to citation-with-paraphrase. Maha paraphrases the source-level result and links to the version of record; no article passage is reproduced. This metadata establishes source identity and inspection scope, not the truth of claims outside the cited locator.
Claims: urn:maha:claim:crispr-cas9-nuclease-editing
Comparison and calculation boundary
Applicability is decided explicitly, not filled with generic material.
This record carries 1 source-bound proposition and therefore has no second supported side. A comparison would have to be manufactured from an adjacent title rather than from a second inspected claim, which the gate forbids.
The canonical claim declares no reproducible numerical inputs, equation, units, or uncertainty propagation; recorded uncertainty kind is qualitative. Supplying sample values would invent an unsupported quantitative result.
Limitations and prohibited inference
The claim stops where its evidence stops.
- record boundary
Biochemical cleavage is not equivalent to efficient, specific, or safe genome editing in a cell or organism.
- record boundary
A source-bounded mechanism, method, or measurement record does not establish manufacturing yield, economic advantage, safety, clinical benefit, or commercial readiness unless those outcomes are measured in a separately scoped record.
- prohibited inference
Do not treat the crispr-cas9 nuclease editing record as medical advice, a treatment recommendation, or evidence of general clinical readiness.
- prohibited inference
Do not transfer a reported result across hardware, organisms, protocols, datasets, operating conditions, or outcome definitions without a declared comparison contract.
- editorial
This compilation reorganizes an existing inspected claim and its declared source; it does not add a new experiment, measurement, or independent replication.
- editorial
Internal editorial inspection is not external peer review, and no result on this page has been independently reproduced.
Related records and mathematical bridges
Typed links expose context without asserting equivalence.
Adenine base editing
Same canonical domain (synthetic-biology). Domain membership only: no shared source or declared edge links these two records.
Selection: domain adjacency
Double-strand-break repair outcomes
Declared mechanistic-dependency edge into this record, so it is positioned earlier in the same bounded sequence.
Selection: bridge edge
Guide RNA and PAM recognition
Declared mechanistic-dependency edge from this record. The edge is navigational and asserts no equivalence or causation beyond the cited source scope.
Selection: bridge edge
When no declared bridge edge is present, related records are linked by shared evidence or canonical domain adjacency. Those links are navigational and do not claim mathematical or physical equivalence.
Connected domain graph
Typed dependencies preserve publication state.
Only independently canonical records receive public links and relation statements. Draft graph topology remains private.
Guide RNA and PAM recognition
outbound connection · mechanism
Target recognition depends on guide complementarity and a compatible adjacent motif.
Guide RNA and PAM recognition
inbound connection · mechanism
Guide and motif recognition direct the nuclease mechanism.
Double-strand-break repair outcomes
inbound connection · mechanism
Nuclease editing delegates the final sequence outcome to cellular break repair.
Claim ledger
Every proposition keeps its own evidence state.
The cited biochemical study reconstitutes programmable Cas9 cleavage and shows that a designed single guide can direct sequence-specific cutting adjacent to a compatible motif.
- Scope
- The constructs, biological systems, protocols, assays, datasets, and comparisons reported in A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
- Boundary
- Biochemical cleavage is not equivalent to efficient, specific, or safe genome editing in a cell or organism.
- Uncertainty
- There is no universal effect estimate for this method; numerical results remain attached to the source experiment, biological system, assay, and analysis choices.
- Replication
- This candidate records one bounded source package. Independent replications and contradictory results must be compiled separately before evidence maturity is upgraded.
Primary sources
Citation, locator, rights, and boundary travel together.
Source 1 · Science
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity
Martin Jinek, Krzysztof Chylinski, Ines Fonfara, Michael Hauer, Jennifer A. Doudna, Emmanuelle Charpentier
- Exact locator
- Abstract; Figures 1–5; supplementary materials describing guide design and cleavage assays.
- Establishes
- The study reconstitutes RNA-programmed Cas9 cleavage in vitro and shows that a designed single-guide RNA can direct sequence-specific DNA cleavage adjacent to an appropriate motif.
- Boundary
- An in-vitro nuclease mechanism does not establish editing efficiency, specificity, delivery, safety, or phenotype in cells or organisms.
- Rights basis
- citation with paraphrase · Maha paraphrases the source-level result and links to the version of record; no article passage is reproduced.