Bounded definition
The cited study develops genome-scale CRISPR knockout libraries and reports positive and negative selection screens in specified human cell models. Within this page, that proposition is limited to The constructs, biological systems, protocols, assays, datasets, and comparisons reported in Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells.
Definition and evidence boundary
Parallel genetic perturbation using a guide library, selectable phenotype, and sequencing-based guide abundance readout. The bounded proposition retained by the canonical record is: The cited study develops genome-scale CRISPR knockout libraries and reports positive and negative selection screens in specified human cell models.
The applicable scope is The constructs, biological systems, protocols, assays, datasets, and comparisons reported in Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells. This definition must not be generalized beyond the cited source and exact record boundary.
Claims: urn:maha:claim:pooled-crispr-screening
Mechanism and technical context
The study develops a pooled lentiviral CRISPR knockout library and applies positive and negative selection screens in specified human cell models. This is the source-bound technical context for the record; no uncited mechanism is added by the compiler.
Guide enrichment identifies screen-dependent associations and can reflect editing, growth, delivery, copy number, sampling, and model-specific effects. The mechanism or method is therefore presented as one component of a larger system, not as evidence for every downstream outcome.
Claims: urn:maha:claim:pooled-crispr-screening
How to interpret the evidence
There is no universal effect estimate for this method; numerical results remain attached to the source experiment, biological system, assay, and analysis choices. The evidence maturity recorded here is single study, and the claim kind is empirical claim.
This candidate records one bounded source package. Independent replications and contradictory results must be compiled separately before evidence maturity is upgraded. Screen enrichment is model-, library-, delivery-, coverage-, phenotype-, and analysis-specific and is not direct clinical or organism-level evidence. These qualifications travel with the claim whenever it is reused.
Claims: urn:maha:claim:pooled-crispr-screening
What the source supports and what remains unknown
The inspected source supports exactly this: The study develops a pooled lentiviral CRISPR knockout library and applies positive and negative selection screens in specified human cell models. It was read at Abstract; Figures 1–4; supplementary methods; library and screen-analysis sections.
What remains unknown is everything outside that locator. Guide enrichment identifies screen-dependent associations and can reflect editing, growth, delivery, copy number, sampling, and model-specific effects. No quantity, comparison, or downstream outcome is established here unless a separately scoped record measures it.
Claims: urn:maha:claim:pooled-crispr-screening
Source identity, locator, and reuse boundary
The bound source is “Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells” by Ophir Shalem, Neville E. Sanjana, Ella Hartenian, Xi Shi, et al., published by Science on 2013-12-12; its declared stable identity is doi:10.1126/science.1247005.
The inspected-content locator is Abstract; Figures 1–4; supplementary methods; library and screen-analysis sections. Reuse is limited to citation-with-paraphrase. Maha paraphrases the source-level result and links to the version of record; no article passage is reproduced. This metadata establishes source identity and inspection scope, not the truth of claims outside the cited locator.
Claims: urn:maha:claim:pooled-crispr-screening
Comparison and calculation boundary
Applicability is decided explicitly, not filled with generic material.
This record carries 1 source-bound proposition and therefore has no second supported side. A comparison would have to be manufactured from an adjacent title rather than from a second inspected claim, which the gate forbids.
The canonical claim declares no reproducible numerical inputs, equation, units, or uncertainty propagation; recorded uncertainty kind is qualitative. Supplying sample values would invent an unsupported quantitative result.
Limitations and prohibited inference
The claim stops where its evidence stops.
- record boundary
Guide enrichment identifies screen-dependent associations and can reflect editing, growth, delivery, copy number, sampling, and model-specific effects.
- record boundary
A source-bounded mechanism, method, or measurement record does not establish manufacturing yield, economic advantage, safety, clinical benefit, or commercial readiness unless those outcomes are measured in a separately scoped record.
- prohibited inference
Do not treat the pooled crispr screening record as medical advice, a treatment recommendation, or evidence of general clinical readiness.
- prohibited inference
Do not transfer a reported result across hardware, organisms, protocols, datasets, operating conditions, or outcome definitions without a declared comparison contract.
- editorial
This compilation reorganizes an existing inspected claim and its declared source; it does not add a new experiment, measurement, or independent replication.
- editorial
Internal editorial inspection is not external peer review, and no result on this page has been independently reproduced.
Related records and mathematical bridges
Typed links expose context without asserting equivalence.
Adenine base editing
Same canonical domain (synthetic-biology). Domain membership only: no shared source or declared edge links these two records.
Selection: domain adjacency
Cell-free gene expression systems
Same canonical domain (synthetic-biology). Domain membership only: no shared source or declared edge links these two records.
Selection: domain adjacency
Single-cell perturbation readout
Declared mechanistic-dependency edge from this record. The edge is navigational and asserts no equivalence or causation beyond the cited source scope.
Selection: bridge edge
When no declared bridge edge is present, related records are linked by shared evidence or canonical domain adjacency. Those links are navigational and do not claim mathematical or physical equivalence.
Connected domain graph
Typed dependencies preserve publication state.
Only independently canonical records receive public links and relation statements. Draft graph topology remains private.
Single-cell perturbation readout
outbound connection · measurement
Single-cell readouts add state-resolved measurements to pooled perturbation designs.
Single-cell perturbation readout
inbound connection · measurement
Perturb-seq extends pooled screens with cell-resolved transcriptomic outcomes.
Claim ledger
Every proposition keeps its own evidence state.
The cited study develops genome-scale CRISPR knockout libraries and reports positive and negative selection screens in specified human cell models.
- Scope
- The constructs, biological systems, protocols, assays, datasets, and comparisons reported in Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells.
- Boundary
- Guide enrichment identifies screen-dependent associations and can reflect editing, growth, delivery, copy number, sampling, and model-specific effects.
- Uncertainty
- There is no universal effect estimate for this method; numerical results remain attached to the source experiment, biological system, assay, and analysis choices.
- Replication
- This candidate records one bounded source package. Independent replications and contradictory results must be compiled separately before evidence maturity is upgraded.
Primary sources
Citation, locator, rights, and boundary travel together.
Source 1 · Science
Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells
Ophir Shalem, Neville E. Sanjana, Ella Hartenian, Xi Shi, et al.
- Exact locator
- Abstract; Figures 1–4; supplementary methods; library and screen-analysis sections.
- Establishes
- The study develops a pooled lentiviral CRISPR knockout library and applies positive and negative selection screens in specified human cell models.
- Boundary
- Screen enrichment is model-, library-, delivery-, coverage-, phenotype-, and analysis-specific and is not direct clinical or organism-level evidence.
- Rights basis
- citation with paraphrase · Maha paraphrases the source-level result and links to the version of record; no article passage is reproduced.